Finding reliable sealing solutions for 96-well plates is essential for accurate PCR runs, sample containment, and data integrity. The following selections focus on durable sealing films and mats that offer easy application, strong adhesion, and compatibility with common laboratory workflows. Each option below is designed to minimize leakage, reduce evaporation, and maintain sample integrity across standard 96-well formats.
| Product | Brand | Notes |
|---|---|---|
| PCR Plate Sealing Film | Scienfocus lab | 130 x 80 mm, easy tear, strong seal |
| 96-Well PCR Plate Silicone Cap | MUHWA | Silicone sealing mat, reusable |
| PCR Plate Sealing Film | Feleolibe | 83×133 mm, 100 sheets |
| BrandTech 701352 Deep Well Plate | BrandTech | 1.1 mL, 96 wells, round bottom |
| KIESEV 96 Well Plate Sealing Film (Opaque) | KIESEV | 130 x 80 mm, 100 sheets |
Overview: The table above highlights five products that align with common sealing needs for 96-well formats, including self-adhesive films, silicone mats, and deep-well plate options. Each product is selected for clear adhesion, compatibility with standard PCR workflows, and practical packaging for regular laboratory use. The choices cover both disposable sealing films and reusable silicone solutions to accommodate different lab preferences and budgets.
PCR Plate Sealing Film – 96-Well Plates (Scienfocus lab)
Product overview: Scienfocus lab offers 130 x 80 mm sealing film designed for 96-well PCR plates, with an easy tear opening suitable for gloved use. The film emphasizes strong viscosity after pressing, ensuring a tight seal with minimal residual adhesive. It is described as high-transparency to allow clear inspection of contents and is designed to prevent leakage across wells. These features support routine PCR workflows with reduced risk of evaporation during thermal cycling.
96-Well PCR Plate Silicone Cap Mat (MUHWA)
Product overview: MUHWA provides a silicone sealing mat sized for 96-well PCR plates. The mat is commonly used to seal wells during assays that require repeated access or temporary sealing between steps. It’s described as non-sterile, with a compact footprint (approximately 11.8 cm by 7.8 cm) and is suitable for educational settings and research laboratories. Silicone mats are known for reusability and reliable sealing when used with appropriate PCR plate lids or cap mats.
100 Sheet PCR Plate Sealing Film – 96 Well (Feleolibe)
Product overview: Feleolibe offers a sealing film designed for 96-well plates, with a standard 83×133 mm size and 100 sheets per package. The film is self-adhesive and transparent, promoting straightforward application and visual monitoring of contents. It is marketed for typical laboratory PCR workflows, emphasizing reliable sealing and ease of use in busy lab environments.
BrandTech 701352 Deep Well Plate (Polystyrene)
Product overview: BrandTech offers a polystyrene deep-well plate with 96 wells, each well having a 1.1 mL volume and a round bottom shape. While primarily a plate to seal, its deep-well design supports high-volume assays and complex sample handling. The material choice and well geometry are compatible with standard sealing films and mats, providing versatility for various assay formats and storage needs.
KIESEV 96-Well Plate Sealing Film (Opaque & 100 Sheets)
Product overview: This KIESEV sealing film is designed for a variety of plate formats, including PCR plates. It features self-tearing, high transparency, high viscosity, and minimal residual adhesive. The film is not temperature resistant and may deform above 80°C, so it should not be used with high-heat PCR instruments. The 130 x 80 mm size and pack of 100 sheets suit routine sealing tasks in standard labs seeking dependable self-adhesive performance.
KIESEV 96-Well Plate Sealing Film (Transparent & 100 Sheets)
Product overview: This transparent KIESEV film is self-tearing for easy use, with high viscosity and no residual glue. It shares 130 x 80 mm dimensions and a 100-sheet pack, making it a practical option for consistent sealing across numerous plates. Not recommended for high-temperature applications due to potential deformation beyond 80°C.
KIESEV 96-Well Plate Sealing Film (Transparent & Numbered)
Product overview: This variant from KIESEV offers a transparent, self-tearing 130 x 80 mm sealing film with numerical identifiers for easy tracking. The film emphasizes high visibility of plate contents and reliable adhesion, suitable for diverse lab workflows. Like other non-thermally resistant films, avoid high-temperature exposure to prevent deformation.
Buying Guide
Key purchase considerations
- Plate compatibility: Ensure the sealing film size (130 x 80 mm or similar) matches your 96-well plate format and lid design.
- Adhesion and seal quality: Look for products that emphasize strong viscosity, minimal residual glue, and minimal leakage post-sealing.
- Material and reuse: Silicone mats offer reusability, while sealing films are disposable. Consider lab throughput and budget when choosing.
- Temperature tolerance: If your workflow involves thermal cycling, verify the film’s suitability for the instrument and cycling temperatures.
- Transparency and inspection: High transparency facilitates visual checks of wells for condensate, bubbles, or leaks during and after sealing.
- Ease of use: Tear-open features and compatibility with gloved hands can impact efficiency in busy labs.
- Packaging and quantity: Larger packs reduce reorder frequency, but initial needs should guide quantity to avoid waste.
Comparative considerations: Sealing films excel for single-use, low-cost workflows and straightforward storage. Silicone mats are advantageous when reuse is feasible and where repeated sealing cycles are common. Deep-well plates offer higher volumes per well and can adapt to varied assay formats, but require compatible seals and lids. Always align the choice with your instrument compatibility, lab protocol, and workflow speed to optimize results and reliability.
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